Month: October 2007

It’s over!

No you can't have $10,000. Not yours.

I am proud to announce that I am 5th Loser in this 2007 College Blogging Scholarship competition!

Lacking the emotional appeal and/or existing promotional network of the top scorers, I was pretty much up the creek without a plunger. Given the popularity contest format of the competition, I’m actually pretty pleased with how I did. My regular readers (judging by the hits to the RSS feed) have approximately tripled or quadrupled, and I did get a small but useful amount of feedback to help improve things. Oh, and hey, I seem to have readers in Berlin and somewhere in Chile, among other places, so now I can say I’m “world famous™”. Though the proportion of voters who actually did check out all of the blogs was pitifully low, it does still look like it was around 1-2% of the voters, which is actually higher than I would have predicted.

I get the impression that some of us running less well known blogs were a little disappointed about the format of the competition, but there’s really no reason to be. All it means is that rather than being a contest for “highest quality” blog, it was a contest for “most effective” blog. Certainly, being able to get your “vote for me” message out to a larger range of people is a valid measure of effectiveness, so the results seem reasonable to me. And I wasn’t the bottom scorer. Judging by the way my score moved, at least some portion of the people who were examining all of the blogs actually did like what they saw here as I was getting a couple of votes a day on average, so I’m doing something right at least.

The only complaint I really have about the “popularity contest” format is this: I think one of the major benefits to humanity of “blogging” is the fact that unlike mainstream media, a blogger can afford to present unusual, less broadly popular content which otherwise would never be made available. Not having to worry about the internet equivalent of “Nielsen Ratings”, we can afford to put up obscure or strange things that only a fraction of the world might be interested in, which is why if you poke around the internet, you can find something that isn’t the latest celebrity crap or badly-reported political scandal. I actually don’t know how much of a role it played in this particular competition, but this sort of approach in general strikes me as something that would be strongly biased towards “mainstream” content. I think a little more love for all of us off-center folks would be in order.

I also hope they’re offering runner-up prizes again this year. Even if *I* don’t win, at least one of “my people” (nerds, that is – hey, you don’t go for a PhD in Neuroscience without being at least a little bit of a nerd…) would get something again this year if they do.

This does mean, though, that I won’t have $10,000 to buy a microscope with. Woe is me. On the other hand, that means I’ve got no excuse not to try begging in front of scientific conferences. I figure that ought to be worth some entertainment, once I get some time to try it. Perhaps by this time next year, I’ll have a bit more fame and popularity and have a better shot at the prize.

Hey, scienceblogs.com, if you want to promote my blog next year when I’m (hopefully) in graduate school, I may have a shot at the prize next time around… (UPDATE: It may not be obvious, but this should be read as good-natured jealously, not some kind of complaint or accusation…)

And now that all that’s over, we’ll be returning once again to my usual nerdity. Stay tuned (some more).

Give a man a fish, and you feed him for a day…

…but teach a man to fish, and he’ll sit in a boat and drink beer all day.

-== We interrupt this blog post to bring you this important announcement: Happy Birthday, Dad!==-

(His birthday was actually yesterday, but this week of school has been grinding me pretty hard and I’d forgotten all about it. He must be so proud – his son can handle a couple of semesters of biochemistry, complex microbial science, working with dangerous chemicals in a lab…but doesn’t seem to know how to use a simple calendar…I am filled with shame.)

We now return you to your regularly scheduled blog post:

This here critter is our resident fish. “He” is a classic specimen of real, old-fashioned, Honest-to-Aquaman Carassius auratus auratus – the Goldfish. And not one of those poor mutant freaks who can barely swim, either. No this here fish was rescued from the overcrowded “feeder goldfish” tank of a local Wal-Mart®. Handsome, ain’t he? I had a tough time getting even this good of a picture – every time I get near the tank he swims back and forth in front of me frantically, perhaps worshipping me as the magical fishfood god. He’s been here for about three years now, so I think he’s having a much longer life than most of them.

I’ve had no time to get into it, but part of the reason for having a fish is that I have a casual interest in aquaculture. That is, while I don’t currently have any intention of becoming a professional full-scale fish-farmer, the subject is interesting and, I think, very important in the near future. Once we figure out where we’re going to end up living next year and get settled in somewhere, I have considered trying to do the aquaculture equivalent of a backyard garden, though.

I think aquaculture is going to become extremely important in the relatively near future, as we run into the combination of overfishing of natural stocks, water shortages, contamination of natural waters with pollutants that build up in naturally-existing populations of fish, and the overall effects of climate change. I think understanding how to raise healthy and nutritious aquatic food without wasting water or causing environmental problems is going to be a useful set of knowledge to have. (There, see, not only do I love kittens and puppies and want to make the world a better place, but I’m also interested in Sustainable Environmental Practices™. While feeding the hungry. [Uphill. In the snow. With no shoes…]).

You may be wondering what interest an ex-professional-computer-nerd microbiologist would have in tending a pond full of eukaryotes. Well, aside from the obvious “Hey, I can have more than one interest, you know”, there actually is a lot of microbiological activity involved in the natural processes of the fishes’ homes. Plus, of course, the aforementioned beer doesn’t ferment and bottle itself, you know.

Since one of my interests in this context is water conservation, my main interest is in figuring out how to maintain a healthy “closed” system. In an aquaculture context, a “closed” system is one that you don’t normally add substantial amounts of water to. (An example of an “open” system might include raising fish in pens floating in a natural lake, or having a constant stream of fresh ground or river water pumping through your tanks). This poses certain problems, since you have to feed the fish, and this adds an ever-increasing load of potentially uneaten fishfood and especially of eaten fishfood – that is, fish wastes.

Fishfood being digested by either fish or bacteria ends up adding ammonia to the water, which is poisonous to the fish (and crawdads and whatever else is in there). Also excreted is carbon dioxide, which makes the water more acidic, and unused food also dumps sulfur and phosphorous into the system.

If you’ve ever had a fishtank, you may know about the ammonia. Certain kinds of Oxygen-using bacteria can actually get some of their biochemical energy from turning reduced nitrogen into oxidized nitrogen, ultimately turning the ammonia (NH3) into much less poisonous nitrate (NO3). These bacteria tend to colonize the tank’s filter, where they do their thing using the oxygen in the water that flows through. Even nitrate is dangerous if it builds up too much, though. In an aquarium, they usually recommend just taking out some of the tank’s water and replacing it with fresh water every week or two to get rid of the build-up. I’d show you pictures of the bacteria, but I still can’t afford a decent microscope. (sniffle.)

Anyway, I want to build a denitrification column one of these days. There are bacteria that can “breathe” nitrate in place of oxygen, and in the process they can reduce the nitrate back down to plain old harmless nitrogen gas, which just bubbles out of the water. If you build a long, tall tube full of something like gravel that bacteria can grow on, and then pump the water through it slowly, oxygen-breathing bacteria near the bottom of the tube rapidly use up the oxygen in the water, leaving the nitrate. With no oxygen further up the tube, bacteria that can breathe the nitrate instead can grow like crazy, and exhale the extra nitrogen out of the system.

That’s one way of avoiding the need to use up as much fresh water as you’d need if you relied only on replacing the water to get rid of the nitrate.

I’ll save the sulfur and phosphorous parts for another day. Meanwhile, I think the next podcast or two will deal with MRSA, since it’s been in the news so much lately. I normally find the neglected non-medical microbiology more interesting, but the biochemistry and genetics involved with Methicillin Resistant Staphylococcus Aureus (not to mention S. aureus itself) is pretty interesting, and I find the media discussions of it unsatisfying.

Stay tuned…

Hello, College Blogging Scholarship reviewer and other casual viewers

I see the hits from people examining the finalist blogs (including this one) at the 2007 College Blogging Scholarship are up, presumably since this is the last weekend of voting (insert obligatory “please vote for me” plea here).

I have a favor to ask of you, and everyone else who happens to stumble on this blog one way or another (including my regular readers): Please tell me something about your impression of this blog. Even if all you have time for is a quick one-sentence comment, praise for something you like or thoughtful criticism of something you don’t like, or just something that you thought was noteworthy, it will help me improve the blog. No registration is necessary to comment.

If you have time for a more detailed comment, some opinions as to what else you might be interested in seeing here would be helpful. For example, I’m considering trying to do a regular or semi-regular podcast. Would that be of interest? More pictures? More detailed discussions of scientific matters? Naked pictures of myself? (Okay, almost none of you would really want the latter…)

I’m actually more interested in your opinions than your votes, though if I can have both I would obviously be grateful…

I shall return again to the science nerdity intended for a broad not-necessarily-nerdy audience shortly. Thank you.

#1 on Google!

Over on scienceblogs.com’s The World’s Fair, the author has started an amusing meme.

It goes like this: the challenge is to find 5 sets of search terms for which your own blog or site is the #1 hit on a Google search. Note that it is acceptable to quote specific phrases but of course it’s more impressive if you don’t. Here are 8 that (as I type this) for which this blog is the #1 hit (links go to the blog address that is the hit):

There was at least one other which I’m having trouble remembering at the moment. Perhaps I’ll update later if I remember what it was.

My server’s going to walk funny for a week after this…

Someone posted a link to the “No, You Can’t Have A Cookie” image I put together a while back.

On Fark.com.

In a comment thread for an article that seems to involve the suggestion of a nude college girl.

The server logs have been scrolling by rapidly for quite a while now. Ow.

Incidentally, if you’re coming here from Fark.com, do me a favor and click on the “vote for me” image thing below. I promise if I win, I’ll use some of the money to get a TotalFark account…

UPDATE: The thread seems to be FINALLY winding down. Thank you, Farkers who noticed my plea and voted for me, I’m rapidly gaining on the 4th Loser position!

Hey, it beats last…and voting goes through Sunday this week, as I recall…

Microbial Fuel Cell netcast…

It’s only my first attempt at anything like this, so constructive comments are welcome…

(Hopefully you can see the embedded audio player here…)For those of you just tuning in, this is a 90-second explanation of Why Microbial Fuel Cells work. A longer (though still simple) explanation can be found at a slightly older post here.[Update: this was featured in the November 6, 2007 broadcast! Hooray, I can now claim to be an international “radio personality”!]Presuming hosting this file doesn’t kill my bandwidth, I’ll leave it up here. BelowAbove, you should see an embedded flash player (assuming you have Macromedia® Flash® player installed) which you should be able to click on to start the audio. I’ll also place a direct download link below. It should be noted that like everything else on this blog (unless otherwise specified), this audio is also available under the Creative Commons non-commercial/attribution/share-alike license, so as long as you have no problem with the terms of that license you are welcome to copy, redistribute, put up on bittorrent, host a public performance, turn into an interpretative dance art project, or whatever else you might want to do with it so long as you give me credit for it, don’t use it for commercial purposes, and distribute any derivative works of it under the same terms.

You can download the audio directly from here – right-click on the link and select “save link as…”. Ogg Vorbis format available on request…

Poor-boy science: should I build my own electrophoresis platform?

I want to build my own little electrophoresis gizmo to play with.

I did pick up a small tube of powdered graphite and some liquid tape. With this, I should be able to make a waterproof electrically-conducting glue that I can use for the electrodes. I’ve got numerous old “wall-wart”-type power adapters that I ought to be able to use for power supply.

The main thing I’m trying to work out in my head before I start trying to actually put this together is exactly how I’m going to arrange it so that I can have either a thin gel or a piece of paper or other fibrous material in between the electrodes so that I can best separate things.

I suppose it’s kind of bizarre, but this is actually part of the ongoing Expired JellO® projects. I was wondering to myself what actual changes might possibly occur in a packet of dry gelatin mix over time, and how would I be able to tell?  My previous experiments have shown no indication that there are any easily detectable differences (no obvious changes in taste or texture, no strange eerie glow, no acquisition of superpowers upon eating it…) so I’ll have to look more closely.

It occurred to me that just maybe over time the strands of protein that make up gelatin might get damaged by oxidation from the air in the pouch (or do they seal the pouch in a relatively inert gas, like argon or nitrogen?). This isn’t something one can really tell just by looking, obviously. One MIGHT be able to tell indirectly by making fresh and “expired” packets of gelatin with the same precisely-measured amount of water, poured on at the same precisely-measured temperature, and ideally with the same amount of mixing. Believe it or not, there are actually special scientific devices for measuring the firmness of gels like this. The hypothesis would be that expired gelatin might end up “degraded” into smaller strands of protein than a fresh packet, and that this would be reflected in a reduced firmness of the gel, or perhaps reduced water-holding capacity.

However, I don’t have access to precise devices for measuring things like that, and in any case since I suspect the difference would be pretty minimal, I’m not sure any difference in firmness would really be detectable with any kind of instrument I could cobble together on my own. What to do?…

I thought that if I had a way to subject a sample of dissolved gelatin to electrophoresis, I could then use a protein-staining substance to see how broad of a range of protein-fragment sizes were existent, or perhaps even spot distinct fragments if oxidative damage tended to happen at the juncture between particular amino acids or something.

I’m not quite sure why, but I have a strong desire to do this experiment from scratch as a “hillbilly biotech” exercise (including building the equipment and obtaining my supplies from grocery or hardware stores rather than specialty scientific supply places).

There are special protein staining compounds I can use at the end to see where my bits of protein ended up after electrophoresis. “Coomassie Brilliant Blue“, for example, but they don’t have that down at the grocery store. (And if you think that’s a funny name for a dye, consider “Light Green SF Yellowish”…)

Then, I ran into a post indirectly about henna over on scienceblogs.com. It seems the natural orange-staining ingredient in henna, called lawsone, may be specifically a protein-staining substance. I’m not certain about this, but a dark-orange protein-staining dye would work for my purposes I think. If so, that solves my need to get a protein stain from an ordinary store.

It’ll be a little while before I can try to put this plan into action, but I think I’ll be able to get to it in the next month or two.

In other news, I think I’ll try to post my “Microbial fuel cells in 90 seconds” audio sometime tomorrow. Then I can work on more. Anybody want to hear me attempting to explain something in 90 seconds? So far I’ve considered MRSA, and perhaps how cow flatulence threatens the world’s climate (which is also a microbiological topic). I’m sure there must be plenty of other possible topics. Any suggestions?

P.S. Who wants audio in Ogg Vorbis format in addition to mp3?

A short update…

I’ve got an Art History exam in the morning which has been consuming my time, but I wanted to get some kind of post up. Especially since it almost looks as though I just don’t post on Thursdays. I swear the recent several weeks of “no post on Thursday” is purely coincidental.

I’m still waiting to hear if This Week In Science got the audio file I sent them and whether or not they liked it. I did manage to find a legally-free embeddable flash-based audio player that I can use, so I’ll probably post it for listening to online or for downloading soon.

Meanwhile: One of my competitors in the College Blogging Scholarship 2007 competition had an interested post up the other day. Famous neuroscientist Shelley Batts of Retrospectacle posted about a bunch of computer people getting together to have a “hackfest”, where they all work on their projects and exchange ideas. She wonders if something similar might not be possible for scientists.

I have regularly found myself thinking about the possibility of a similar gathering for scientists. I wonder – would an international society of Peripatetic Scientists be feasible? What I envision would be a combination of “Science Cafe’” and “Semi-spontaneous field trip” (or even perhaps a “Flash Mob of Nerds”).

I picture groups of scientists, engineers, and other interested people converging on relatively short notice (say, no more than a week or so) to explore something together, whether it’s a section of a national park, or an observatory, or a grocery store, or even to just wander around in a public space discussing some topic. Rather than a carefully planned and organized event where people take turns “giving presentations”, I tend to suspect a more spontaneous exploration by a group of diverse people like this would result in much better horizontal meme transfer potential. It’s so much easier to participate and listen when one isn’t busy focusing on one’s own presentation material…

How many of you reading this might be interested in participating in this sort of thing?
(UPDATE: TO clarify, I mean how many of you, if you heard something like this was happening where you are, would be interested, not how many people are so incredibly impressed by me that they would travel across the world to be where I am…)

Electricity-breathing bacteria! (Microbial fuel cells)

I made a 90-second “pod”cast of why microbial fuel cells work. I don’t yet know if This Week in Science is or was interested in playing it. [Update: this was featured in the November 6, 2007 broadcast! Hooray!] Either way, once I find a way to make it available without killing my bandwidth I shall. I’ll probably do more of them – if nothing else I obviously need the practice.

It was oddly difficult getting myself to actually talk to the microphone – more so than actually publically speaking to real people. I’m not sure why. It strikes me as something I’ll get over quickly once I’ve done it a few times, and my voice won’t sound quite so bland in the future.

In any case, microbial fuel cells are possibly the topic that got me really interested in a college education in applied biotechnology. I’ve been meaning to do a post on why they work for a while, so here’s one, in somewhat more detail than the 90-second audio version.

First, some quick review: We all remember that atoms are made of positively-charged protons, uncharged neutrons, and negatively-charged electrons, right? Protons and usually neutrons in the middle, and electrons hovering around. When atoms chemically react with each other, they’re really just having a fight over who gets to keep the electrons. When the reaction is over, some kinds of atoms or groups of atoms will have gained at least partial custody of electrons that used to belong to some of the other atoms or groups of atoms. The ability of a kind of atom to take electrons away from other atoms is called “electronegativity”. The second most electronegative element in the universe just happens to be a major part of our atmosphere – Oxygen.

As bacteria break down food molecules to get biological energy, there are electrons left over along the way. The bacterial cells have specific carrier molecules that take these extra electrons away, where they can be later dumped elsewhere into any of a variety of other useful biological reactions that need them. The one we’re concerned with today is called the Electron Transport Chain.

In many bacteria, and in the mitochondria of plants, fungi, and animals, the Electron Transport Chain regenerates a huge amount of a cell’s biochemical energy. The extra electrons get sucked into the beginning of this chemical chain, and as they are pulled along, the force of this pull drives a process which regenerates the cells’ main energy-carrying molecule, called ATP. This process is “respiration”, and it’s also exactly the reason you need to breathe oxygen. Humans need so much energy just to remain alive that we couldn’t survive without the huge amount of extra energy that respiration provides.

What drives this whole chain is some chemical at the other end pulling the electrons out. In aerobic organisms, this is oxygen. Some bacteria can use other chemicals, like nitrates, sulfates, and ferric iron (yes, there are bacteria that can breathe rust…) None of these chemicals provide quite as much energy as oxygen does, but it’s better than nothing and gives bacteria that could be damaged by oxygen something to breathe.

Normally, this last step happens inside the cell, but some bacteria have ways of extending this last step so that the final hand-off of the electrons happens outside itself. Some bacteria even make electrically-conducting biological “nano-wires” that this can happen through. Others make “shuttle” molecules that can pick up electrons, dissolve out of the cell, hand off the electrons somewhere outside, and then dissolve back into the cell to pick up more.

Now, we can make a microbial fuel cell. An electrode is put where the bacteria are growing – without oxygen – and a wire runs from this, out of the area where the bacteria are and to another electrode which is exposed to oxygen. It’s like an electric snorkel for bacteria. From the electrode and through the wire, the oxygen sucks electrons away from the bacteria. An electrical device stuck between the ends of the wire can use this energy exactly the same way that it could use the energy from electrons being sucked from one end of a battery to another.

Interestingly, the common “simple stain” Methylene Blue can also act as an artificial “shuttle” molecule. When reduced (carrying extra electrons) methylene blue is actually colorless, and I would swear I’ve seen protocols somewhere that use this to measure just how active a yeast culture is, and one of the demonstration microbial fuel cell setups actually uses a culture of yeast in methylene blue rather than a microbe that can naturally breath through electrodes.

By the way, if you thought you could tell a human from a realistic humanoid robot bent on world domination by the fact that only humans eat, I’ve got bad news for you. One interesting application of microbial fuel cells is Gastrobots. Literally, robots with digestive systems, where bacteria breaking down the contents of the “stomach” act as a microbial fuel cell to power the robot.

I hope you find this explanation useful and interesting. If you have (or even if you haven’t) please let me know. I can’t necessarily tell if I’m doing anybody any good without feedback!

Stir-fried random…

Just a few brief random comments for the moment:

  • Am I the only one who is already completely sick and tired of the word “spooky”?
  • I think I’ve figured out what Descartes’ problem is. He’s gone on this meditation where he’s convinced himself that as far as he knows, nothing exists…except for himself. I think what happens next is that he gets horribly lonely, so when he realizes that his thoughts also exist with him, that’s when he developed that unwholesome passion for them and inability to bear leaving them that I’d previously mentioned. “But what kind of thing am I? I’m a thing that thinks. A thinking thing is what I am. But what kind of thing is that? Oh, yeah, I already said, it’s a thinking thing. Did I mention I was a thinking thing that thinks thoughts?….”. Okay, Descartes, we got it the first time…
  • One of my fellow “college science bloggers whose obscurity currently keeps them low in the vote totals” actually has a pretty neat blog. The Biourbanist focusses on features and attributes of urban areas. Well worth adding to your RSS feeds, I think. After you’ve already voted for me, of course…
  • I am currently attempting to put together my first netcast, in which I shall attempt to crunch an explanation of why microbial fuel cells work, in a form hopefully comprehensible to anyone with a good junior-high-school science education (or a mediocre high-school education, which is probably sadly more common), that fits into 90 seconds. Wish me luck.

More to follow…
UPDATE: Got the “pod”/netcast done – a real blog post on the subject of Microbial Fuel Cells to go with it may be found here tomorrow (Tuesday, October 16th) sometime, so long as nothing unexpected happens…